Explanation:
In glycolysis, ATP is first needed to supply inorganic phosphate, which is attached to the glucose molecule to form a phosphorylated sugar molecule, fructose 6-phosphate. ATP is needed again to supply a second inorganic phosphate which is attached to this already phosphorylated sugar molecule to form fructose 1,6-bisphosphate.

Explanation:
A second infection by a pathogen triggers a much faster and more potent immune response, resulting in the production of greater levels of antibodies (Choice B), when compared to primary infection. Primary infection causes a smaller increase in antibody numbers; this is represented by Y (Choice A). Infection by a different pathogen (Choice C) would cause a raise in antibody numbers similar to levels in point Y. Antibiotics do not increase antibody numbers (Choice D).

Explanation:
Choice B is correct because Rubisco is the name of the enzyme which is responsible for the fixation of carbon during photosynthesis. Choice A is incorrect as rhodopsin is a pigment found in the retina which is responsible for detecting light . Choices C and D are incorrect as neither are enzymes, with collagen being a structural component of tissues such as skin and insulin being a hormone responsible for regulating blood glucose levels.

Explanation:
Glycolysis occurs in the cytoplasm of the cell. Then, in aerobic respiration, the product of glycolysis (pyruvate), then is transported into the mitochondrion, where it enters the link reaction. The link reaction takes place in the fluid part of the mitochondrion, the matrix. The product of the link reaction then enters the Krebs cycle, which also occurs in the matrix. The final stage, the electron transport chain, occurs in the membranes of the cristae. Choice B is, therefore, correct.

Choices A, C and D are incorrect as they give one or more incorrect locations.

Explanation:
Photosynthesis (Choice B) is the process by which carbon dioxide in the air is converted to carbon compounds in green plants.

Respiration releases carbon dioxide (Choice A), and decomposition by decomposers (Choice C) also releases carbon dioxide. Transpiration is the process by which water is drawn up the stem of a plant to the leaves where it evaporates (Choice D) and is not involved directly in the carbon cycle.

Explanation:
The statement for Choice B is incorrect, because longitudinal muscle does not stop the bolus from moving backwards, this is the role of the circular muscles. Choice A, Choice C and Choice D are correct statements because the longitudinal and circular muscles do work together, longitudinal muscle does move the bolus along the alimentary canal and the bolus is mixed with enzymes as a result of peristalsis.

Explanation:
A positive feedback mechanism is a physiological process in which a response amplifies the initial stimulus, leading to an accelerated and often self-reinforcing outcome. The dilation of the cervix during childbirth stimulates the release of the hormone oxytocin. Oxytocin then brings about further uterine contractions, which brings about further cervix dilation. This is, therefore, an example of positive feedback, and Choice D is correct.

A negative feedback mechanism is a regulatory mechanism in which a response counteracts and reverses the initial stimulus, maintaining stability and homeostasis in the system. Choices A, B, and C are all examples of negative feedback mechanisms.

Explanation:
The mass of enzyme added remains the same throughout because the enzyme acts as a catalyst and does not get used up during the reaction. All other choices (Choices A, B and C) are used in obtaining the rate of an enzyme-catalysed reaction since they involve measuring the substrate used or product formed over a specified amount of time.

Explanation:
In glycolysis (Choice A), glucose is broken down into pyruvate and this happens in the cytoplasm. Glycolysis is incorrect because pyruvate is only produced at the end of glycolysis and is not converted to acetyl CoA.

Pyruvate produced during glycolysis enters the mitochondria where it is converted to acetyl CoA during the link reaction. (Choice B)

The Link reaction (Choice B) is the reaction that bridges glycolysis with the next stage which is the Krebs cycle (Choice C). Hence, the Krebs cycle is incorrect because acetyl CoA is the reactant and needs to be produced prior to entering the Krebs cycle.

The stage after the Krebs cycle is known as oxidative phosphorylation (Choice D) which involves the synthesis of ATP.

Explanation:
Thin-layer chromatography is a method that can be used to separate pigments extracted from a leaf. Paper chromatography uses paper with a line drawn approximately 1 cm from one end, which is called the baseline (Choice D). This is where a drop of leaf extract is placed. Then the paper is immersed in a solvent with the level of solvent being right below the baseline. This solvent will then move up the paper to separate the pigments found in the leaf extract.

The coloured spots show the distance moved by each pigment (Choice C). The solvent will then move further up and will eventually stop moving. The mark where the solvent stops is known as the solvent front and shows the distance moved by the solvent (Choice B).

In order to find the Rf value for a given pigment, the distance moved by the pigment is divided by the distance moved by the solvent (Choice A).

Explanation:
When organisms respire, they lose energy in the form of heat. This form of energy cannot be recovered and is finally lost from the system.

Initially, during photosynthesis, energy is captured from light (Choice A). Carbon dioxide is not a form of energy (Choice C), and chemical energy (Choice D) is held in the bonds of organic molecules made during photosynthesis.

Explanation:
In the light-dependent reaction, NADP is the final electron acceptor in Photosystem I and this is reduced to form NADPH.

CO22 is fixed by the enzyme Rubisco and is not an electron acceptor (Choice A).

ATP is synthesized by ATP synthase using the proton gradient in the thylakoids and is not an electron acceptor (Choice C).

Oxygen (Choice B) is the terminal electron acceptor in aerobic cell respiration, but not in photosynthesis.

Explanation:
Choice A - Cells of multicellular organisms have different functions. They interact with one another to perform more complex functions such as responding to the environment, movement and homeostasis.

Choice B - Cells of multicellular organisms communicate with each other. These cells communicate to coordinate the activities of organs, tissues and other cells.

Choice C - Cells interact with one another forming complex structures and functions that any one cell type alone could not perform

Choice D - Cells have different structures based on the cell’s specialised roles.

Explanation:
A respirometer is a device that is used to measure the rate of respiration. A basic feature of a respirometer is a sealed container containing the living organism and an alkaline substance such as soda-lime (Choice A) or potassium hydroxide to absorb the carbon dioxide gas, which is the product of respiration.

The rate of respiration is measured by a reduction in the volume of oxygen due to the oxygen consumed by the living organism. The reduction in the volume of oxygen inside the sealed container causes a reduction in pressure inside the container. This causes the syringe to move which is how oxygen consumption is measured (Choice B). The rate of respiration can then be calculated based on the amount of oxygen consumed over a period of time.

An oxygen gas sensor (Choice C) can also be used to measure the reduction in the amount of oxygen. However, the experimental setup for this question does not include a sealed bio chamber bottle that is designed to fit the gas sensor probe. Since this experimental setup used a bung to seal the container, removing it to place an oxygen gas sensor probe inside the flask will not provide an accurate measurement of the amount of oxygen consumed.

When using a respirometer, the temperature is a controlled variable and should be kept constant (Choice D) to avoid changes to the volume of oxygen gas. Temperature also affects the rate of respiration. There should not be more than one independent variable being tested in an experimental setup.

Explanation:
Choice C is correct, as ATP is short for adenosine triphosphate. As the name tells us, it has three phosphate groups, represented by the three circles attached to the central ribose sugar. The rectangle represents the adenine.

Choice A is a cholesterol molecule, Choice B is a glucose molecule, and Choice D is a molecule of ADP (adenosine diphosphate), which is the molecule that results when ATP loses its third phosphate molecule, releasing energy.

Explanation:
Aquatic ecosystems take in inorganic carbon when carbon dioxide dissolves in water. Dissolved carbon dioxide can combine with water to form carbonic acid (H22CO33) which dissociates into hydrogen ions (H

) and hydrogen carbonate ions (HCO33

). So, inorganic carbon is present as both dissolved carbon dioxide and hydrogen carbonate ions in aquatic ecosystems.

Carbon dioxide, not carbon monoxide (Choice B) is the form of inorganic carbon that autotrophs can use. Methane (Choice C and D) is a form of organic carbon, not inorganic carbon.

Explanation:
Choice C is correct because smallpox was the first human disease eradicated by vaccination. Choice A is incorrect because polio has not been fully eradicated. Choice B is incorrect because monkeypox has not been eradicated. Choice D is incorrect because there is no vaccine for bubonic plague.

Explanation:
Choice D is correct because the gland X is the pineal gland and it produces melatonin. Choice A is incorrect because leptin is produced by adipose tissue. Choice B is incorrect because thyroxine is produced by the thyroid gland. Choice C is incorrect because progesterone is produced by ovaries.

Explanation:
Cells are the basic structural and functional units of life. They are the fundamental building blocks of all living organisms. Tissues are groups of specialised cells that work together to perform a specific function. Different types of tissues combine to form organs. Organs are composed of multiple tissues that work together to carry out complex functions. Organs have specific roles within the body. Organ systems are groups of organs that collaborate to perform broader functions necessary for the survival of the organism. Organism refers to the complete living individual made up of various organ systems working together. Therefore Choice B is correct.

Choices A, C and D are all in the incorrect order.

Explanation:
Choice D is correct because cAMP binds to inactive PKA after being formed to activate PKA.

Choice A is incorrect because the enzyme adenyl cyclase (AC) acts on ATP to form cAMP. This has to take place first to form cAMP. Choices B and C are incorrect because these two reactions take place before cAMP is formed. A ligand such as epinephrine binds to the G protein-coupled receptor, resulting in guanosine triphosphate (GTP) binding to the G protein to activate it.

Explanation:
Photolysis is the splitting of water molecules in the presence of light to produce electrons, hydrogen ions and oxygen. This is part of the light-dependent reaction of photosynthesis.

Chemiosmosis (Choice B) happens in both photosynthesis and cellular respiration. In both processes, ATP is formed from ADP and inorganic phosphate. Hence, the products are not electrons, hydrogen ions and oxygen molecules.

The Calvin cycle (Choice C) is part of the light-independent reaction of photosynthesis. It produces carbon compounds and does not require light.

The electron transport chain (Choice D) is a component of both photosynthesis and cellular respiration. In photosynthesis, it takes place after photolysis which is when water molecules are split, producing electrons, hydrogen ions and oxygen. Photolysis needs to take place first before these electrons can move down the electron transport chain.

Explanation:
Almost all enzymes are globular proteins and differ from fibrous proteins in that they are sensitive to pH and temperature and are mostly water soluble.

Fibrous proteins (Choice A), unlike globular proteins, are long and narrow, less sensitive to changes in pH and temperature and are generally insoluble in water. Enzymes are not lipids (Choices B, D). Enzymes tend to be hydrophilic, not hydrophobic since they are mostly soluble in water.

Explanation:
Neonicotinoids bind irreversibly to acetylcholine receptors at cholinergic synapses so that acetylcholine (a neurotransmitter) is unable to bind and an action potential cannot be generated.
Choices B, C and D are incorrect as they do not describe how neonicotinoids work.

Explanation:
Cell respiration is the process where energy is released from organic compounds to form adenosine triphosphate (ATP). ATP consists of the nitrogenous base adenine, a ribose sugar and three phosphate groups. These three components contain the elements oxygen, hydrogen, phosphorus, carbon and nitrogen (Choice D). Even though ATP is made up of these five elements, they are not products of hydrolysis.

ATP is the immediately available source of energy in cells and fuels cell processes. This takes place when ATP undergoes hydrolysis and the energy which is stored in the phosphate bond of ATP is released. The phosphate bonds that are broken lead to the formation of adenosine diphosphate (ADP), inorganic phosphate and a release of energy.

Hydrolysis is a catabolic reaction of breaking down large molecules into small molecules. Water is required to split apart ATP, hence both water and ATP are the reactants in a hydrolysis reaction and not products (Choice C).

The main stages of cellular respiration are glycolysis, the Krebs cycle and the electron transport chain. In glycolysis, glucose is broken down to pyruvate (Choice A) which then moves on to the Krebs cycle and electron transport chain before generating a high yield of 38 ATP.

Explanation:
Both, the brewing of beer and baking of bread, involve anaerobic yeast fermentation. In the brewing of beer, yeast feeds on glucose and respires anaerobically to produce ethanol, carbon dioxide and energy. The ethanol produced is the alcohol found in beer. Similarly, with the baking of bread, yeast respires anaerobically to produce ethanol, carbon dioxide and energy. However, the ethanol that is produced evaporates during baking.

Carbon dioxide (Choice A) is a product present in both the brewing of beer and the baking of bread. Carbon dioxide is soluble in beer and is found in small amounts. It is responsible for the rising of the bread.

Likewise, water (Choice D) is also a substance present in both the brewing of beer and the baking of bread. In the brewing of beer and in the baking of bread, water is added to the mixture.

In addition to yeast fermentation, anaerobic respiration can also occur in humans and other animals. This is known as lactic acid fermentation. Glucose is not broken down completely and forms lactic acid (Choice B) in the absence of oxygen.