Explanation:
The digestion of starch is a catabolic reaction where starch molecules are broken down into maltose by hydrolysis.

Peptide formation from amino acids (Choice A), starch synthesis from glucose (Choice B), and DNA synthesis from nucleotides (Choice D) are all examples of anabolic reactions.

Explanation​:
Since the experiment is about testing the temperature; the temperature would be the independent variable, not the controlled variable. A water bath would be used in this experiment to set up different increments of the independent variable.

When investigating the effect of temperature on photosynthesis, the other limiting factors (concentration of carbon dioxide and light intensity) must be controlled. The light intensity can be controlled by placing the test tube containing the Elodea at a constant distance from the same light source for each trial (Choice C) to make sure that the light intensity is the same for all trials.

Using the same volume and concentration of sodium hydrogen carbonate solution (Choice B and Choice D)ensures that the source of carbon dioxide is controlled, and it is not a limiting factor.

Explanation​:
An ecosystem refers to an area where organisms interact with each other and with the physical environment.

A habitat (Choice A) refers to the environment in which a species normally lives. A population (Choice C) is a group of organisms of the same species living in the same area at the same time which can interbreed. A community (Choice D) is a group of populations living and interacting with each other in the same habitat.

Explanation​:
Choice A is correct because all statements are true. Plasma cells are clones of B lymphocytes, produce large numbers of antibodies and have extensive rough endoplasmic reticulum.

Explanation:
All the statements above are true. In most ecosystems, light energy from the Sun is captured by producers via photosynthesis and transferred to consumers through feeding. As organisms respire, they lose energy in the form of heat which cannot be reused by organisms. There is a constant input of energy from the Sun.

Explanation:
Okapi is a herbivore (II) because it only eats plants. Okapi is a species (III) since it can interbreed and produce fertile offspring.

They are not a hybrid of a giraffe and a zebra (I), even though they look like one.

Explanation:
Choice A is correct because zoonotic diseases are diseases that can be transmitted from one species to another, such as from chickens to humans. Choice B is incorrect because zoonotic diseases do not only affect domesticated animals. Choice C is incorrect because zoonotic diseases are not only caused by viruses. Choice D is incorrect because zoonotic diseases do not only affect humans.

Explanation​:
Choice A - Cells of multicellular organisms have different functions. They interact with one another to perform more complex functions such as responding to the environment, movement and homeostasis.

Choice B - Cells of multicellular organisms communicate with each other. These cells communicate to coordinate the activities of organs, tissues and other cells.

Choice C - Cells interact with one another forming complex structures and functions that any one cell type alone could not perform

Choice D - Cells have different structures based on the cell’s specialised roles.

Explanation​:
Almost all enzymes are globular proteins and differ from fibrous proteins in that they are sensitive to pH and temperature and are mostly water soluble.

Fibrous proteins (Choice A), unlike globular proteins, are long and narrow, less sensitive to changes in pH and temperature and are generally insoluble in water. Enzymes are not lipids (Choices B, D). Enzymes tend to be hydrophilic, not hydrophobic since they are mostly soluble in water.

Explanation​:
Aquatic ecosystems take in inorganic carbon when carbon dioxide dissolves in water. Dissolved carbon dioxide can combine with water to form carbonic acid (H22​CO33​) which dissociates into hydrogen ions (H

) and hydrogen carbonate ions (HCO33​

). So, inorganic carbon is present as both dissolved carbon dioxide and hydrogen carbonate ions in aquatic ecosystems.

Carbon dioxide, not carbon monoxide (Choice B) is the form of inorganic carbon that autotrophs can use. Methane (Choice C and D) is a form of organic carbon, not inorganic carbon.

Explanation​:
Glycolysis occurs in the cytoplasm of the cell. Then, in aerobic respiration, the product of glycolysis (pyruvate), then is transported into the mitochondrion, where it enters the link reaction. The link reaction takes place in the fluid part of the mitochondrion, the matrix. The product of the link reaction then enters the Krebs cycle, which also occurs in the matrix. The final stage, the electron transport chain, occurs in the membranes of the cristae. Choice B is, therefore, correct.

Choices A, C and D are incorrect as they give one or more incorrect locations.

Explanation​:
A respirometer is a device that is used to measure the rate of respiration. A basic feature of a respirometer is a sealed container containing the living organism and an alkaline substance such as soda-lime (Choice A) or potassium hydroxide to absorb the carbon dioxide gas, which is the product of respiration.

The rate of respiration is measured by a reduction in the volume of oxygen due to the oxygen consumed by the living organism. The reduction in the volume of oxygen inside the sealed container causes a reduction in pressure inside the container. This causes the syringe to move which is how oxygen consumption is measured (Choice B). The rate of respiration can then be calculated based on the amount of oxygen consumed over a period of time.

An oxygen gas sensor (Choice C) can also be used to measure the reduction in the amount of oxygen. However, the experimental setup for this question does not include a sealed bio chamber bottle that is designed to fit the gas sensor probe. Since this experimental setup used a bung to seal the container, removing it to place an oxygen gas sensor probe inside the flask will not provide an accurate measurement of the amount of oxygen consumed.

When using a respirometer, the temperature is a controlled variable and should be kept constant (Choice D) to avoid changes to the volume of oxygen gas. Temperature also affects the rate of respiration. There should not be more than one independent variable being tested in an experimental setup.

Explanation:
Choice C is correct because smallpox was the first human disease eradicated by vaccination. Choice A is incorrect because polio has not been fully eradicated. Choice B is incorrect because monkeypox has not been eradicated. Choice D is incorrect because there is no vaccine for bubonic plague.

Explanation​:
Cell respiration is the process where energy is released from organic compounds to form adenosine triphosphate (ATP). ATP consists of the nitrogenous base adenine, a ribose sugar and three phosphate groups. These three components contain the elements oxygen, hydrogen, phosphorus, carbon and nitrogen (Choice D). Even though ATP is made up of these five elements, they are not products of hydrolysis.

ATP is the immediately available source of energy in cells and fuels cell processes. This takes place when ATP undergoes hydrolysis and the energy which is stored in the phosphate bond of ATP is released. The phosphate bonds that are broken lead to the formation of adenosine diphosphate (ADP), inorganic phosphate and a release of energy.

Hydrolysis is a catabolic reaction of breaking down large molecules into small molecules. Water is required to split apart ATP, hence both water and ATP are the reactants in a hydrolysis reaction and not products (Choice C).

The main stages of cellular respiration are glycolysis, the Krebs cycle and the electron transport chain. In glycolysis, glucose is broken down to pyruvate (Choice A) which then moves on to the Krebs cycle and electron transport chain before generating a high yield of 38 ATP.

Explanation​:
Photosynthesis is the production of carbon compounds such as glucose using light energy. Oxygen is produced as a waste product which then diffuses out of the cell. Plants use smaller molecules such as carbon dioxide and water to build larger molecules like glucose. Hence, photosynthesis is an anabolic reaction. An anabolic reaction requires energy to build larger molecules. Photosynthesis takes in energy so is known as an endothermic reaction.

Cell respiration (Choices B and D) is the production of ATP, carbon dioxide and water using glucose and oxygen. Glucose is broken down to form smaller molecules so it is categorized as a catabolic reaction. A catabolic reaction releases energy when larger molecules are broken down to form smaller molecules. Reactions that release energy are known as exothermic reactions.

Explanation:
The data collected for recording the presence and absence of two plants can be used in a chi-squared test to determine if the two plants are associated. This is a relevant statistical method.

The biodiversity found in an area (Choice A) is usually determined by using Simpson’s Diversity Index or other similar calculations. The chi-squared test cannot be used to find the range (Choice B) for heights of trees or predict the probability of finding certain plants (Choice C) in an area.

Explanation​:
The endocrine system (Statement II) is involved in the fight or flight response through the release of hormones from endocrine glands, such as adrenaline (epinephrine), from the adrenal glands. Adrenaline (epinephrine) is released into the bloodstream and travels throughout the body to target organs and tissues, preparing the body for immediate action. When adrenaline (epinephrine) reaches its target tissues it binds to specific transmembrane receptors on the surface of cells (Statement I). This binding triggers a cascade of intracellular events that ultimately lead to physiological changes associated with the fight or flight response, such as increased heart rate and dilated airways. The autonomic nervous system (Statement III) plays a central role in the fight or flight response. In a frightening situation, the nervous system is activated, leading to the release of adrenaline (epinephrine), increased heart rate, increased blood flow to muscles, and other physiological changes that prepare the body for a rapid response. Choice D is therefore correct.

Choices A, B and C are all incorrect as they do not include all three statements.

Explanation​:
The active site of an enzyme is specific to a particular substrate. All other statements are true (Choices A, C and D).

Explanation:
Thin-layer chromatography is a method that can be used to separate pigments extracted from a leaf. Paper chromatography uses paper with a line drawn approximately 1 cm from one end, which is called the baseline (Choice D). This is where a drop of leaf extract is placed. Then the paper is immersed in a solvent with the level of solvent being right below the baseline. This solvent will then move up the paper to separate the pigments found in the leaf extract.

The coloured spots show the distance moved by each pigment (Choice C). The solvent will then move further up and will eventually stop moving. The mark where the solvent stops is known as the solvent front and shows the distance moved by the solvent (Choice B).

In order to find the Rf value for a given pigment, the distance moved by the pigment is divided by the distance moved by the solvent (Choice A).

Explanation:
Cells are the basic structural and functional units of life. They are the fundamental building blocks of all living organisms. Tissues are groups of specialised cells that work together to perform a specific function. Different types of tissues combine to form organs. Organs are composed of multiple tissues that work together to carry out complex functions. Organs have specific roles within the body. Organ systems are groups of organs that collaborate to perform broader functions necessary for the survival of the organism. Organism refers to the complete living individual made up of various organ systems working together. Therefore Choice B is correct.

Choices A, C and D are all in the incorrect order.

Explanation​:
ATP from cell respiration is an immediate source of energy for metabolic processes.

Neurons transmit information in the form of electrical impulses (Statement I). This transmission can only happen when there is a difference in concentrations of Na

and K

ions across the axonal membrane of a neuron. Energy in the form of ATP is required for the Na

/K

pump to restore the ionic balance after the voltage-gated response.

Osmosis (Statement II) does not require ATP. It is a passive transport process where water can move through a partially permeable membrane from an area of lower solute concentration to an area of higher solute concentration.

The condensation reaction of a polypeptide (Statement III) is the joining of amino acids together to form a polypeptide. It is an anabolic reaction where ATP is required to join amino acids together to form a peptide bond, and water is produced.

Explanation:
Facilitated diffusion is a passive process that allows the movement of molecules across a cell membrane with the help of transport proteins. It does not require energy input (ATP) because molecules move down their concentration gradient. Therefore Choice B is correct.

Sodium-potassium pumps (Choice A) actively transport sodium ions out of cells and potassium ions into cells against their concentration gradients. This process requires energy in the form of ATP. Endocytosis (Choice C) is an active process by which cells engulf substances by invagination of the cell membrane to form a vesicle around them. This process requires energy in the form of ATP. Anabolic reactions (Choice D) involve the synthesis of complex molecules from more simple ones. These reactions typically require energy input in the form of ATP.

Explanation:
The statement for Choice B is incorrect, because longitudinal muscle does not stop the bolus from moving backwards, this is the role of the circular muscles. Choice A, Choice C and Choice D are correct statements because the longitudinal and circular muscles do work together, longitudinal muscle does move the bolus along the alimentary canal and the bolus is mixed with enzymes as a result of peristalsis.

Individuals of the same species competing for resources (Choice B) is ‘intraspecific Competition.’ Choice A refers to predator-prey interactions and is not any type of competition. Individuals of different species cooperating for limited resources (Choice D) describes a mutualistic relationship, not a competitive one.

Explanation:
Choice D is correct because the gland X is the pineal gland and it produces melatonin. Choice A is incorrect because leptin is produced by adipose tissue. Choice B is incorrect because thyroxine is produced by the thyroid gland. Choice C is incorrect because progesterone is produced by ovaries.