Explanation:
Choice A is correct because zoonotic diseases are diseases that can be transmitted from one species to another, such as from chickens to humans. Choice B is incorrect because zoonotic diseases do not only affect domesticated animals. Choice C is incorrect because zoonotic diseases are not only caused by viruses. Choice D is incorrect because zoonotic diseases do not only affect humans.

Explanation​:
Diversity indices are used to measure the diversity of organisms in an area (Choice A). A t-test (Choice B) is used to see if there is a significant difference between the means of two groups. Chi-square analysis (Choice C) can be used to check for the association of two species after collecting data on the presence or absence of either or both in defined areas. More generally, Chi-square analysis is used to determine if there is a significant difference between observed and expected. Frequency found in quadrats (Choice D) records how frequently one or more species are found in quadrats and is not a statistical test.

Explanation​:
Choice A - Antibodies are globular proteins synthesized by plasma B cells. The genes associated with antibody synthesis are expressed in plasma B cells.

Choice B - Macrophages do not synthesize antibodies and therefore do not express these genes. Macrophages are large phagocytic cells and present antigens to helper T cells.

Choice C - Helper T cells do not synthesize antibodies. They stimulate B cells to divide by mitosis to produce plasma B cells and memory B cells.

Choice D - Red blood cells do not have a nucleus and therefore do not have the genes for antibody synthesis.

Explanation​:
Statements I and III are correct and describe how enzymes interact with their specific substrates. Statement II is incorrect because, in many catabolic reactions, the substrate is larger than the enzyme.

Explanation:
Okapi is a herbivore (II) because it only eats plants. Okapi is a species (III) since it can interbreed and produce fertile offspring.

They are not a hybrid of a giraffe and a zebra (I), even though they look like one.

Explanation:
All the statements above are true. In most ecosystems, light energy from the Sun is captured by producers via photosynthesis and transferred to consumers through feeding. As organisms respire, they lose energy in the form of heat which cannot be reused by organisms. There is a constant input of energy from the Sun.

Explanation:
The data collected for recording the presence and absence of two plants can be used in a chi-squared test to determine if the two plants are associated. This is a relevant statistical method.

The biodiversity found in an area (Choice A) is usually determined by using Simpson’s Diversity Index or other similar calculations. The chi-squared test cannot be used to find the range (Choice B) for heights of trees or predict the probability of finding certain plants (Choice C) in an area.

Explanation​:
A second infection by a pathogen triggers a much faster and more potent immune response, resulting in the production of greater levels of antibodies (Choice B), when compared to primary infection. Primary infection causes a smaller increase in antibody numbers; this is represented by Y (Choice A). Infection by a different pathogen (Choice C) would cause a raise in antibody numbers similar to levels in point Y. Antibiotics do not increase antibody numbers (Choice D).

Explanation:
Choice B is correct because vaccinations involve the deliberate introduction of antigens, which have been made harmless, to stimulate the production of antibodies and memory cells for long-term immunity. They do not contribute to antibiotic resistance.
Increased and incorrect use of antibiotics contributes to antibiotic resistance.
Choice A increases the use of antibiotics in raising livestock.
Choice C is an incorrect use of antibiotics because they are ineffective against viruses.
Choice D can lead to the development of antibiotic resistance because patients who fail to complete a course of antibiotics may not completely eliminate the infection and instead allow the survival of resistant bacteria.

Explanation:
Facilitated diffusion is a passive process that allows the movement of molecules across a cell membrane with the help of transport proteins. It does not require energy input (ATP) because molecules move down their concentration gradient. Therefore Choice B is correct.

Sodium-potassium pumps (Choice A) actively transport sodium ions out of cells and potassium ions into cells against their concentration gradients. This process requires energy in the form of ATP. Endocytosis (Choice C) is an active process by which cells engulf substances by invagination of the cell membrane to form a vesicle around them. This process requires energy in the form of ATP. Anabolic reactions (Choice D) involve the synthesis of complex molecules from more simple ones. These reactions typically require energy input in the form of ATP.

Explanation:
As energy moves from producers to consumers, only about 10% is transferred to the next trophic level. So only about 0.1 % of the energy produced by the producers reaches the tertiary consumers. All other choices (Choice A, B, C) are not correct. Looking at the values in the diagram, producers start with 20 000 units of energy and tertiary consumers receive only 25 units. 25 divided by 20 000 is approximately 0.1%.

Explanation​:
Since the experiment is about testing the temperature; the temperature would be the independent variable, not the controlled variable. A water bath would be used in this experiment to set up different increments of the independent variable.

When investigating the effect of temperature on photosynthesis, the other limiting factors (concentration of carbon dioxide and light intensity) must be controlled. The light intensity can be controlled by placing the test tube containing the Elodea at a constant distance from the same light source for each trial (Choice C) to make sure that the light intensity is the same for all trials.

Using the same volume and concentration of sodium hydrogen carbonate solution (Choice B and Choice D)ensures that the source of carbon dioxide is controlled, and it is not a limiting factor.

Explanation​:
Choice C is correct because antigens are chemicals that stimulate the production of antibodies. Choice A is incorrect because B lymphocytes produce antibodies, not antigens. Choice B is a description of antibodies, which binds to antigens so is incorrect. Choice D is incorrect because hybridoma cells produce antibodies, not antigens.

Explanation​:
Choice A - Cells of multicellular organisms have different functions. They interact with one another to perform more complex functions such as responding to the environment, movement and homeostasis.

Choice B - Cells of multicellular organisms communicate with each other. These cells communicate to coordinate the activities of organs, tissues and other cells.

Choice C - Cells interact with one another forming complex structures and functions that any one cell type alone could not perform

Choice D - Cells have different structures based on the cell’s specialised roles.

Explanation​:
Reaction X represents the synthesis of a more complex compound from simpler molecules and therefore is a depiction of an anabolic reaction.

Reaction X cannot be catabolic (Choice A) since a more complex molecule is produced. Catabolism results in simpler products compared to the reactants. Reaction X cannot represent all metabolic reactions (Choice C) since metabolic reactions include both anabolic and catabolic reactions while reaction X represents only anabolic reactions. Reaction Y represents a catabolic reaction and not an anabolic reaction (Choice D).

Explanation:
Depolarisation of the presynaptic membrane (Statement II) and the resulting influx of calcium ions into the presynaptic neuron (Statement III) cause the exocytosis (release) of neurotransmitters at the presynaptic membrane.

Statement I is false, as the postsynaptic membrane receives neurotransmitters that are released from the presynaptic membrane. Choices A, C and D are incorrect.

Explanation:
Choice C is correct because smallpox was the first human disease eradicated by vaccination. Choice A is incorrect because polio has not been fully eradicated. Choice B is incorrect because monkeypox has not been eradicated. Choice D is incorrect because there is no vaccine for bubonic plague.

Explanation​:
A denatured enzyme has lost its tertiary structure. It is no longer able to function and cannot catalyze reactions.
The active site will not be able to bind to the substrate in most cases (Choice A).
Usually, the sequence of amino acids is not altered. A denatured enzyme has had its three-dimensional structure altered (Choice C).
High temperatures, specifically temperatures above the optimum temperature for a given enzyme cause denaturation and the enzyme can no longer catalyze reactions (Choice D).

Explanation:
The digestion of starch is a catabolic reaction where starch molecules are broken down into maltose by hydrolysis.

Peptide formation from amino acids (Choice A), starch synthesis from glucose (Choice B), and DNA synthesis from nucleotides (Choice D) are all examples of anabolic reactions.

Explanation:
Choice D is correct because the gland X is the pineal gland and it produces melatonin. Choice A is incorrect because leptin is produced by adipose tissue. Choice B is incorrect because thyroxine is produced by the thyroid gland. Choice C is incorrect because progesterone is produced by ovaries.

Explanation:
The statement for Choice B is incorrect, because longitudinal muscle does not stop the bolus from moving backwards, this is the role of the circular muscles. Choice A, Choice C and Choice D are correct statements because the longitudinal and circular muscles do work together, longitudinal muscle does move the bolus along the alimentary canal and the bolus is mixed with enzymes as a result of peristalsis.

Explanation​:
The active site of an enzyme is specific to a particular substrate. All other statements are true (Choices A, C and D).

Explanation:
The length of food chains tends to be shorter than 6 organisms, even including parasites and detritivores. When energy is passed from one organism to the next, most of the energy is generally lost as heat which means that less and less energy is available to higher trophic levels.

All other statements are true.

Explanation​:
Autotrophs are producers and form the base of food chains. They carry out photosynthesis and get their energy from the Sun.

The most abundant organisms (Choice A) are usually the producers but there are exceptions. Energy always enters the food chain via the autotrophs, not consumers (Choice C).

The flow of energy (Choice D) is from producers to primary to secondary to tertiary consumers.

Explanation​:
When organisms respire, they lose energy in the form of heat. This form of energy cannot be recovered and is finally lost from the system.

Initially, during photosynthesis, energy is captured from light (Choice A). Carbon dioxide is not a form of energy (Choice C), and chemical energy (Choice D) is held in the bonds of organic molecules made during photosynthesis.